Genome‐wide identification of transcription factor binding sites with the ChIP‐seq method is an extremely important scientific endeavor − one that should ideally be performed for every transcription factor in as many cell types as possible. A major hurdle on the way to this goal is the necessity for a specific, ChIP‐grade antibody for each transcription factor of interest, which is often not available. Here, we describe CETCh‐seq, a recently published method utilizing genome engineering with the CRISPR/Cas9 system to circumvent the (...) need for a specific antibody. Using the CETCh‐seq method, targeted genomic editing results in an epitope‐tagged transcription factor, which is recognized by a well‐characterized, standard antibody, efficacious for ChIP‐seq. We have used CETCh‐seq in human cancer cell lines as well as mouse embryonic stem cells. We find that roughly 60% of transcription factors tagged using CETCh‐seq produce a high quality ChIP‐seq map, a significant improvement over traditional antibody‐based methods. (shrink)

Because corporate social responsibility (CSR) is potentially beneficial for companies, it is important to understand the factors that improve a company’s CSR practice. Scholars hypothesize that facilitating learning organization characteristics, which are divided in characteristics at the organizational and the operational level, may improve CSR implementation. These characteristics stimulate companies and their members to be critical, learn from the past, and embrace change, but there is limited empirical evidence of this approach. This study addresses this gap by surveying 280 CSR (...) professionals and performing bootstrap mediation analyses to test multiple hypotheses. Learning organization characteristics at the organizational level, play a key role in supporting CSR implementation: leadership for learning, system connection, and group learning show a direct relationship with CSR implementation. It is striking that the role of the learning organization characteristics at the operational level is only indirect; the organizational characteristics mediate their relationship with CSR implementation. (shrink)

The role of the Nobel Laureate Henry Dale (1875-1968) in the history of allergy and the association of anaphylactic conditions with the liberation of histamine is often overlooked. This paper examines his work in this field in the broader context of his researches into endogenous mediators of normal physiological and abnormal pathological functioning. It also assesses the impact of his working environment, especially the unique conditions he enjoyed at the beginning of the twentieth century in the Wellcome Physiological Research Laboratories (...) (WPRL). The WPRL belonged to the pharmaceutical manufacturer Henry Wellcome, and it was the juxtaposition of the routine commercial obligation of testing drugs for Burroughs, Wellcome & Co., with the opportunities of pursuing unfettered physiological research in well-equipped and supported laboratories, that resulted in what Dale referred to as 'happy accidents' when one set of results suggested experimental strategies and designs to another circumstance. In this way an observation of an unusual effect of an extract of ergot of rye led to collaborative chemical and physiological explorations which revealed the presence of histamine, previously known only as a synthetic product. Further work, accidentally facilitated by the fact that the WPRL produced serum anti-toxins commercially and surplus horse serum was used in experiments where other physiologists routinely used saline, hinted that histamine played a role in the symptom complex known as anaphylaxis. This paper explores some of these themes and elaborates their significance. (shrink)

We present a new viable nonlinear chaotic paradigm. This paradigm has four nonlinear terms. The essential features of the new paradigm have been investigated. Our new system is confirmed to have chaotic behaviors by calculating its Lyapunov exponents. The relations of the system states are displayed by a suggested new signal flow graph. The proposed SFG is discussed via some graph theory tools, and some of its hidden features are calculated. In addition, the system is realized via constructing its electronic (...) circuit which helps in the real applications. Also, a robust controller for the system is designed with the aid of a genetic algorithm. (shrink)

Paperback. What has happened to the science of Logic, as an academic discipline and pursuit? The diagnoses do not coincide, but the recognition is growing that since the days of Mill, Russell or Beth, something has gone wrong. A logical vacuum - theoretical as well as practical - in large parts of contemporary culture is easily perceived and is particularly visible in politicis, precisely where logic as a science ought to make its greatest impact.The present volume offers analyses and reflections (...) of various types, written by logicians and other analytical philosophers. Some analyse the logic of political activists and thinkers, or the political thought of a logician. Other chapters offer concrete logico-analytical studies of burning political issues of our time, emphasizing the possibilities for an advancement of logic in, and through parliamentary debate, and of the problems connected with this goal. The final part of the book looks at possib. (shrink)

We consider the question, of longstanding interest, of realizing types in regular ultrapowers. In particular, this is a question about the interaction of ultrafilters and theories, which is both coarse and subtle. By our prior work it suffices to consider types given by instances of a single formula. In this article, we analyze a class of formulas φ whose associated characteristic sequence of hypergraphs can be seen as describing realization of first- and second-order types in ultrapowers on one hand, and (...) properties of the corresponding ultrafilters on the other. These formulas act, via the characteristic sequence, as points of contact with the ultrafilter D, in the sense that they translate structural properties of ultrafilters into model-theoretically meaningful properties and vice versa. Such formulas characterize saturation for various key theories , yet their scope in Keisler’s order does not extend beyond Tfeq. The proof applies Shelah’s classification of second-order quantifiers. (shrink)